ABSTRACT
OBJECTIVE: We aimed to determine whether aerobic training decreases superoxide levels, increases nitric oxide levels, and improves endothelium-dependent vasodilation in the aortas of spontaneously hypertensive rats. METHODS: Spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were distributed into 2 groups: sedentary (SHRsd and WKYsd, n=10 each) and swimming-trained (SHRtr, n=10 and WKYtr, n=10, respectively). The trained group participated in training sessions 5 days/week for 1 h/day with an additional work load of 4% of the animal’s body weight. After a 10-week sedentary or aerobic training period, the rats were euthanized. The thoracic aortas were removed to evaluate the vasodilator response to acetylcholine (10-10 to 10-4 M) with or without preincubation with L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) in vitro. The aortic tissue was also used to assess the levels of the endothelial nitric oxide synthase and nicotinamide adenine dinucleotide oxidase subunit isoforms 1 and 4 proteins, as well as the superoxide and nitrite contents. Blood pressure was measured using a computerized tail-cuff system. RESULTS: Aerobic training significantly increased the acetylcholine-induced maximum vasodilation observed in the SHRtr group compared with the SHRsd group (85.9±4.3 vs. 71.6±5.2%). Additionally, in the SHRtr group, superoxide levels were significantly decreased, nitric oxide bioavailability was improved, and the levels of the nicotinamide adenine dinucleotide oxidase subunit isoform 4 protein were decreased compared to the SHRsd group. Moreover, after training, the blood pressure of the SHRtr group decreased compared to the SHRsd group. Exercise training had no effect on the blood pressure of the WKYtr group. CONCLUSIONS: In SHR, aerobic swim training decreased vascular superoxide generation by nicotinamide adenine dinucleotide oxidase subunit isoform 4 and increased nitric oxide bioavailability, thereby improving endothelial function.
Subject(s)
Animals , Male , Aorta, Thoracic/physiopathology , Endothelium, Vascular/physiopathology , Hypertension/physiopathology , Physical Conditioning, Animal/physiology , Superoxides/analysis , Swimming/physiology , Blotting, Western , Ethidium/analogs & derivatives , Exercise Test , Fluorescence , Hemodynamics , NAD/analysis , NG-Nitroarginine Methyl Ester/analysis , NG-Nitroarginine Methyl Ester/metabolism , Nitric Oxide Synthase Type III/analysis , Nitric Oxide Synthase Type III/metabolism , Nitrites/analysis , Nitrites/metabolism , Random Allocation , Rats, Inbred SHR , Reference Values , Reproducibility of Results , Superoxides/metabolism , Time Factors , Vasodilation/physiologyABSTRACT
Abstract The validity and relevance of research with animals for the development of knowledge in Exercise Science have for long been discussed. Given the complexity of the biological systems, the use of animal models offers a significant contribution to uncover new findings about acute and chronic effects of exercise, particularly when these studies in humans have limitations and ethical implications. There have been notable findings using experimental animals either in basic sciences or in clinical studies involving physiology, pharmacology, genetic, biochemistry, urology, endocrinology and cancer. This article presents a brief review of scientific research using animal models with a focus on exercise training as an effective tool for the prophylaxis and treatment of different pathological processes, which are the basis of many concepts taught and used in undergraduate courses and graduate programs, as well as in new researches showed in scientific conference meetings in numerous areas of science.(AU)
Subject(s)
Humans , Animals , Exercise , Models, Animal , Physical Education and TrainingABSTRACT
A disfunção endotelial apresenta papel de destaque no desenvolvimento e na progressão da aterosclerose por estar diretamente relacionada a alterações na homeostase da parede vascular e no controle da circulação. Estudos clínicos e experimentais têm evidenciado efeitos benéficos do treinamento físico...
Subject(s)
Humans , Endothelium, Vascular , Exercise , Risk Factors , Nitric OxideABSTRACT
O treinamento físico aeróbio (TF) é um importante meio para melhorar a função endotelial. Entretanto, como os vasos se adaptam ao TF ainda não está completamente esclarecido. Desta forma, o presente estudo teve por objetivo investigar, em ratos normotensos, os efeitos do TF sobre a via de produção de óxido nítrico (NO) e a defesa antioxidante vascular, e suas conseqüências sobre a resposta vasodilatadora em aorta isolada. Os ratos foram submetidos a um protocolo de TF aeróbio (esteira rolante, ~55%Veloc. Máx; cinco sessões/sem., 60 min/sessão, período de 11 semanas). Após o TF, foi avaliada a função vasomotora in vitro pela curva de concentração-efeito à acetilcolina (ACh) e ao nitroprussiato de sódio (NPS), e realizadas medidas bioquímicas na aorta. O programa de TF aumentou significativamente (P < 0,05) em 62% a expressão da enzima óxido nítrico sintase endotelial (eNOS). Entretanto, o TF não modificou significativamente a expressão e atividade da enzima antioxidante superóxido dismutase.Além disso, o TF não modificou o relaxamento individual e a sensibilidade à ACh. Por outro lado, o TF diminuiu significativamente a sensibilidade ao NPS (-8,26 ± 0,081 vs. -7,79 ± 0,099 Log [M], S vs T, respectivamente, P < 0,001). Os resultados apresentados demonstram que o TF aeróbio foi capaz de alterar um dos mecanismos envolvidos na bioatividade do NO, marcadamente o aumento da expressãoda eNOS. Entretanto, esta modificação não levou à melhora da responsividade vasodilatadora aórtica estimulada pela acetilcolina e provocou menor sensibilidade ao NPS.
Aerobic training (AT) is an important way to improve endothelial function. However, it is not completelyunderstood how the blood vessels adapt themselves to the AT. Therefore, the aim of this study was to investigate exercise training-induced adaptations on the nitric oxide (NO) production, antioxidant defense and aorta vasodilatation in normotensive rats. The rats were subjected to an AT protocol (treadmill,~55% Max Veloc., 5 bouts/week, 60 min/bout, 11 wks). After the AT, it was examined in vitro vasomotor function to acetylcholine (ACh) and sodium nitroprusside (SNP), and biochemical analysis in the aorta. Aerobic training significantly increased (P < 0.05) by 62% the expression of the endothelial nitric oxide synthase (eNOS). However, ET did not modify the relaxation response and sensitivity to ACh. In contrast, AT significantly reduced aortic sensitivity to SNP (-8.26 ± 0.081 vs. -7.79 ± 0.099 Log [M], Sed vs. AT,respectively, P < 0.001). These results demonstrate that aerobic AT was able to modify one important mechanism related to the NO bioactivity, which is the increase of eNOS expression. However, this response did not contribute to improve of the aortic vasodilatation response to acetylcholine and decreased the sensitivity to SNP.
Subject(s)
Animals , Male , Rats , Aorta , Physical Conditioning, Animal/physiology , Nitric Oxide , Rats, Wistar , Superoxide Dismutase , Vasodilation/physiologyABSTRACT
El diagnóstico exacto y precoz del origen de la hemorragia digestiva baja es la condición que nos permitirá considerar con resultados favorables un tratamiento acorde a la etiología de este episodio en donde podrán incluirse procedimientos médicos, endoscópicos o quirúrgicos. El colon es el lugar de mayor frecuencia de hallazgos (80 por ciento); se debe tener en cuenta que el 40 por ciento de estos tienen origen orificial, donde predomina la patología hemorroidaria. Menos frecuente (9 por ciento) se observan en patologías de intestino delgado. Se debe tener en cuenta que apróximadamente el 75 por ciento a 85 por ciento de las HDB dejan de sangrar en forma espontánea. Y que la hemorragia masiva o copiosa constituye el 1.5 por ciento de una emergencia quirúrgica. Existe también episodios de sangrado digestivo alto que puede manifestarse como HDB (11 por ciento).